Fig. 4.

Pyruvate prevents the enhancement of CtBP recruitment to E-cadherin by hypoxia. H1299 human lung cancer cells were pretreated with 30 mM pyruvate to prevent the NADH increase and were assayed by ChIP using an anti-CtBP antibody. Primers surrounding the proximal E-boxes in the E-cadherin promoter (E-cadherin) were used to PCR-amplify the ChIP sample. Human LINE1 serves as a nonspecific control.