Fig. 1.

Expression of LTβ pathway genes in developing mouse hair follicles. (A) In situ hybridization of an LTβ-specific probe. Broad expression in epidermis at E13.5 (Upper Left), high expression in hair germ (arrow) and pregerms (arrowheads) at E15.5 (Upper Center), and in the matrix region of maturing hair follicles (arrowheads) at E18.5 (Upper Right) in WT mice. (Lower Right) Weak expression in Ta hair pegs only at E18.5. (Lower) No signal from a control sense probe (WT-s). Dotted lines demarcate epidermis and hair pegs from the dermis. (B) NF-κB expression. (Left) RelB protein localized by immunohistochemistry in cytoplasm of basal layer of epidermis both in WT and Ta and highly expressed in guard hair germs at E14.5. (Right) p65 highly expressed throughout epidermis in WT and Ta. (Scale bars: 50 μm.)