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. 2006 Jun 6;103(24):9148–9153. doi: 10.1073/pnas.0602800103

Fig. 3.

Fig. 3.

Evidence of functionality of IFN-AR2-F8S amino acid substitution. (a) Details of relative quantification of transcript levels in heterozygotes for the IFN-AR2 gene. Values used are the RNA expression level divided by the genomic DNA level to control for relative assay efficiency. The data are shown as plots describing the mean ± 2 SE. All data were analyzed by using a Student’s t test after testing for normality of data (version 12; SPSS, Chicago). (b) FACS analysis of cell surface receptor expression. The gating threshold is set at the level exceeded by <5% of untransfected cells, and the proportion of cells exceeding the threshold is given in each case. IFN-AR2 expression in U5a cells transfected with either IFN-AR2-F or IFN-AR2-S. (c) IFN-AR2 expression of EBV-transformed B cell lines of known genotypes. (d) MHC class I expression induced by IFN-α2b in U5a cells expressing either allele of the IFN-AR2 gene and controls. (e) Antiviral responses induced by IFN-α2b in U5a cells expressing either allele of the IFN-AR2 gene and controls.