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. 2006 Jun 6;103(24):9148–9153. doi: 10.1073/pnas.0602800103

Fig. 4.

Fig. 4.

Evidence of functionality of IL-10RB-K47E amino acid substitution. (a) Details of relative quantification of transcript levels in heterozygotes for the IL-10RB gene. Values used are the RNA expression level divided by the genomic DNA level to control for relative assay efficiency. The data are shown as plots describing the mean ± 2 SE. All data were analyzed by using a Student’s t test after testing for normality of data (version 12; SPSS, Chicago). (b) Density plots showing FACS analysis of cell surface receptor expression. The gating threshold is set at the level exceeded by <5% of untransfected cells, and the proportion of cells exceeding the threshold is given in each case. IL-10RB expression on the cell surface of Huh7 cells transfected with either IL-10RB-K or IL-10RB-E. (c) IL-10-mediated inhibition of LPS-induced TNF-α expression by IL-10RB-K47E genotype. The concentration of IL-10 used was 5 ng/ml shown with the corresponding percentage inhibition (mean ± 2 SE). At maximum inhibition, KK vs. KE/EE shows a significant difference (P = 0.015). Mean percentage inhibition observed for each genotype were as follows (% ± 2 SE): KK, 74.0 ± 6.7; KE, 83.2 ± 4.7; and EE, 90.5 ± 5.8.