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. 2006 Jun 6;103(24):9166–9171. doi: 10.1073/pnas.0600240103

Fig. 1.

Fig. 1.

Soluble IL-15Rα augments IL-15-mediated lymphocyte proliferation in vitro. (A) Purified MP (CD44hi) CD8+ T cells from IL-7 tg mice were labeled with CFSE and cultured at 5 × 104 cells per well with 5 ng/ml of IL-15. As indicated, 1 μg/ml of either sIL-15Rα-Fc (dimers) or sIL-15Rα (monomers) was added to the cultures. CFSE dilution was assessed on day 4. Representative data are shown. (B) Purified MP CD8+ T cells were cultured with either titrated amounts of IL-15 plus a fixed concentration of soluble receptor (1 μg/ml) (Upper) or titrated amounts of soluble receptor plus a fixed concentration of IL-15 (10 ng/ml) (Lower). The data show mean levels of [3H]thymidine incorporation (±SD) for triplicate cultures on day 3. (C) Purified naïve (CD44lo) CD8+ T cells, MP CD8+ T cells, NK cells, or MP CD4+ T cells were cultured with IL-15 as indicated. Soluble IL-15Rα-Fc was added at 1 μg/ml. CFSE dilution was assessed on day 3. (D) Same as in B except 10 μg/ml of anti-CD122 antibody was added as indicated. (E) MP CD8+ T cells from wild-type IL-7 tg (Ly5.2) and IL-15Rα–/–/IL-7 tg (Ly5.1) mice were mixed together, labeled with CFSE, and cultured as indicated. CFSE dilution on Ly5.1– (wild type) and Ly5.1+ (IL-15Rα–/–) cells was measured on day 3.