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. 2006 Jun 1;103(24):9178–9183. doi: 10.1073/pnas.0602888103

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© 2006 by The National Academy of Sciences of the USA

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Fig. 4. — Western blot analysis of CAP-18 in stool extracts from healthy, Shigella-infected and butyrate-treated rabbits with the CAP-18-specific polyclonal antisera. Lanes 1 and 2 show a marker and the synthetic CAP-18 peptide, respectively. A single band corresponding to the pro-CAP-18 protein appears in the stool of healthy (lanes 3 and 4) and Shigella-infected (lane 5) rabbits 1 day postinfection. Two immunoreactive bands are visualized at day 2 postinfection (lane 6), showing presence of both pro-CAP-18 and active CAP-18 peptides in stool. Butyrate treatment resulted in release of the active CAP-18 peptide in stool (lanes 7–10); day-1 and day-2 stool specimens (lanes 7 and 8) also contained the pro-CAP-18 protein.