Fig. 6.

Immunotherapy-induced anti-MICA antibodies mediate complement-dependent lysis. 293T and 293T-MICA embryonic kidney cells were incubated in patient sera and complement, and cell viability was determined with a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay after 6 h. (Upper) Prevaccination sera. (Lower) Postvaccination sera. M35 was a vaccinated, long-term surviving metastatic melanoma patient (7 years) without anti-MICA antibodies. The differences were abrogated by heat inactivation of the complement.