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. 2006 Jun 5;103(24):9202–9207. doi: 10.1073/pnas.0603095103

Fig. 1.

Fig. 1.

ERAP1−/− mouse embryonic fibroblast lines are defective for peptide trimming in the ER. Fibroblast cell lines were prepared from ERAP1+/+ (black bars) and ERAP1−/− (gray bars) embryonic mice. (A) Cells were stained with mAb directed against H-2Kb (mAb B8.24.3) or H-2Db (28.14.8S) and analyzed by flow cytometry. (B) MEF cells were transfected with plasmids expressing SIINFEKL as a cytosolic ubiquitin fusion (S8L), ovalbumin protein targeted to the cytosol (Ova), LEQLE-SIINFEKL as a cytosolic ubiquitin fusion (N5-S8L), or ALEQLE-SIINFEKL targeted to the ER by a signal sequence (ss.N5-S8L). After 48 h, the cells were stained with mAb 25.D1.16 (anti-H-2Kb-SIINFEKL) and analyzed by flow cytometry. Data (representative of five experiments) show the mean fluorescence intensity with background staining by an irrelevant antibody subtracted (averages of three independent cell lines; error bars represent 1 SD). Asterisks indicate statistically significant differences (P < 0.05, Student t test) between ERAP1+/+ and ERAP1−/− MEF cell lines.