Fig. 4.
CD8+ T cell responses to LCMV are altered in ERAP1−/− mice. ERAP1+/+ (black bars) or ERAP1−/− (gray bars) mice were infected with LCMV. Eight days later, splenocytes were stimulated with peptides corresponding to LCMV epitopes, permeabilized, and stained with anti-IFNγ antibody (A) or stained with tetramers consisting of H-2Kb or H-2Db complexed with the indicated LCMV epitopes and analyzed by flow cytometry (B). Shown is the percentage of CD8+ T cells reactive with each peptide (averages of three mice; error bars represent 1 SD; representative of at least three experiments). Asterisks indicate statistically significant differences (P < 0.05, Student t test) between ERAP1+/+ and ERAP1−/− mice. (Inset) Same data as in A and B, arranged to show the normal and altered immunodominance hierarchies.