Fig. 5.

Altered CD8⁺ T cell responses in ERAP1^−/− mice are not due to altered T cell repertoire. Splenocytes from LCMV-immune B6/SJL mice were adoptively transferred into ERAP1^+/+ (black bars) or ERAP1^−/− (gray bars) mice. One day after transfer, recipient mice were infected with LCMV, and the recall CD8⁺ T cell response generated by the donor cells was analyzed by intracellular IFNγ staining. Shown is the percentage of CD8⁺ CD45.1⁺ T cells producing IFNγ in response to each peptide (averages of three mice; error bars represent 1 SD; representative of two experiments). Asterisks indicate statistically significant differences (P < 0.05, Student t test) between ERAP1^+/+ and ERAP1^−/− mice.