Fig. 1.

Strategy for PfNT1 disruption by double crossover. (A) Gene disruption strategy. I, schematic representation of WT PfNT1 chromosomal locus; II, the plasmid vector pKEΔPfNT1 used for stable transformation of P. falciparum; III, the proposed model of integration of the plasmid by double crossover into the PfNT1 chromosomal locus. (B) Southern blot analysis of XbaI (X) and XbaI plus HindIII (X+H) digested genomic DNA from WT and pfnt1Δ parasites by using BSD-specific and PfNT1-specific probes (as indicated by the gray double-headed arrows in part III of A).