Table 1.
Force, pN | Steps within a backward run* |
First backward step after a forward step† |
||||
---|---|---|---|---|---|---|
Rate constant λ1, s−1 | Rate constant λ2, s−1 | Amplitude ratio Z2/Z1 | n | Rate constant, s−1 | n | |
3 | 11.9 ± 2.6 | 1.7 ± 0.7 | 1.04 | 395 | 1.6 ± 0.2 | 78 |
5 | 17.4 ± 3.3 | 2.6 ± 1.4 | 0.64 | 675 | 2.6 ± 0.3 | 82 |
10 | 35.5 ± 5.6 | 3.8 + 5/−3.7 | 0.03 | 198 | 6.3 ± 2.4 | 20 |
*Data include experiments with 0, 0.25, and 1 μM ATP (displayed in Fig. 3D).
†Data include experiments with 0.25 and 1 μM ATP. The rate constant is calculated as the inverse of the average dwell time.