Fig. 2.

miR-1, miR-133, and miR-206 induction is specific to myogenesis. (A) C₂C₁₂ cells were grown in GM or 2% horse serum (DM) in the presence or absence of 300 ng/ml BMP2. RNA from treated cells were harvested at the indicated times and probed for miR-1, miR-133, miR-206, and U6 small RNAs. Mature and precursor microRNAs are labeled with an arrow and arrowhead, respectively. (B) The same RNA samples used in A was DNase I treated and subject to RT-PCR for the detection of a myogenic marker (myogenin) or a osteoblastic marker (osteocalcin) and a housekeeping gene (GAPDH). Separate RT-PCR reactions were performed but were all loaded in the same well for simultaneous detection by using ethidium bromide staining. RT reactions performed without reverse transcriptase yielded no signal after PCR, and these samples are loaded in the “−” lanes.