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. 2006 May 25;103(23):8780–8785. doi: 10.1073/pnas.0603313103

Fig. 2.

Fig. 2.

Inhibition of LINE-1 retrotransposition and HIV-1ΔVif infectivity by APOBEC3 proteins. (A) The effect of APOBEC3 proteins on HIV-1ΔVif infectivity was quantified as described in ref. 41. Data are presented relative to a culture lacking any APOBEC3 protein (positive), which was designated as 100% activity. Data are the average of at least three experiments, with standard deviation indicated. The relative expression of each APOBEC3 protein was comparable (Fig. 6). Because the effect of APOBEC3 proteins on the infectivity of Vif-deficient HIV-1 was measured by using 293T cells, although their effect on LINE-1 retrotransposition was measured in HeLa cells, these results are only qualitatively comparable. (B) Representative experiment showing the relative number of G418-resistant colonies obtained after selection of HeLa cells transfected with the LINE-1 retrotransposition indicator construct pJM101/L1.3 or the negative (NEG) control construct pJM105/L1.3 (lacking a functional ORF2 gene) in the presence or absence (POS) of the indicated APOBEC3 proteins.