Fig. 3.

Mutation of residues near H539 further define the dsRNA binding site. (A and B) Residues mutated on the concave and lateral surfaces proximal to H539 are highlighted in green and blue, respectively. The two residues important for function, H539 and N541, are colored magenta. The boxed area in A is shown in detail in B. (C) Transfected cells were examined for responsiveness to pI:pC as in Fig. 2A. Bars are color-coded as in A. Only N541A showed significant loss of responsiveness (∗, P < 0.05).