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. 2006 May 23;103(23):8792–8797. doi: 10.1073/pnas.0603245103

Fig. 4.

Fig. 4.

H539E and N541A fail to bind ligand. TLR3-ECD (WT or mutant) protein was incubated with p(I):p(C) or buffer and analyzed by size-exclusion chromatography. Column fractions were assayed for TLR3-ECD by ELISA at 450 nm. Data are representative of two separate experiments. Elution profiles of WT (Top), H539E (Middle), and N541A (Bottom) TLR3-ECD protein are shown. Open bars, protein alone; filled bars, protein plus p(I):p(C). Arrows indicate the elution volumes of pI:pC and TLR3-ECD when run alone.