Fig. 3.
Specific binding of mAbP and mAbM BR55-2 to LeY-overexpressing cancer cells. A total of 1 μg/ml purified mAbM and mAbP BR55 was incubated on 96-well ELISA plates seeded with LeY-positive SK-BR3 and SW948 cells or LeY-negative A431 and WM115 cells (106 per well). CO17-1A, EpCAM antigen-specific mAb; WT, 1 μg of total protein extract from nontransgenic tobacco.