Figure 2.

DAH partitions into the Triton X-114 detergent phase. (A) The membrane pellets were resuspended in 1% Triton X-114 solution and subjected to cloudy point precipitation. Equal volumes of the membrane suspension (T), the aqueous phase (A), and the detergent phase (D) were loaded onto a gel for Western blotting. The gel was probed with DAH and actin antibodies. The detergent phase after the first phase separation (D₁) was washed two more times to give rise to A₂ and D₂, and A₃ and D₃, respectively. The majority of the DAH protein in the membrane fraction partitions into the detergent phases (lanes 3, 5, and 7). (B) Crude embryonic extracts (0–1 and 2–3 h) were prepared for Triton X-114 phase separation, and >60% of the DAH protein is in the detergent phase (lanes 3 and 6).