Table 1.
Primers used for MSP, RT-PCR, and cloning and sequencing.
| MSP primers: | ||
| SLIT1-MF2 | 5'-TtcgTtcgcgagTTagacg-3' | 19 bp |
| SLIT1-MR2 | 5'-aAAcgccgtcgcttAAaAA-3' | 19 bp |
| SLIT1-UF2 | 5'-TgggTttgTgTgTggTgTTT-3' | 20 bp |
| SLIT1-UR2 | 5'-ttttcctcctcAcaAcaAtcaA-3' | 22 bp |
| SLIT2-MF | 5'-gggaggcgggattgTTTag-3' | 19 bp |
| SLIT2-MR | 5'-catAAcgcgcgAAAAtAcac-3' | 20 bp |
| SLIT2-UF | 5'-gTgggaggTgggattgTTTa-3' | 20 bp |
| SLIT2-UR | 5'-AcctctccctcAccctcAac-3' | 20 bp |
| SLIT3-MF | 5'-ggtttcgtcgatggagttgt-3' | 20 bp |
| SLIT3-MR | 5'-aaacgcgtaaaacccgaaa-3' | 19 bp |
| SLIT3-UF | 5'-TGTGggTTagTGgggTTagg-3' | 20 bp |
| SLIT3-UR | 5'-cacaaacaaaacaaaacactcca-3' | 23 bp |
| ROBO1-MF2 | 5'-cggcggcgatagTagTTaaa-3' | 20 bp |
| ROBO1-MR2 | 5'-cgAAActAAAAAcgcccaAa-3' | 20 bp |
| ROBO1-MF3 | 5'-cggcgtgcgTTTTTaTaatg-3' | 20 bp |
| ROBO1-MR3 | 5'-gccAcgAAtAAcccgctAct-3' | 20 bp |
| ROBO1-UF | 5'-TggTggTaaagttggggtgt-3' | 20 bp |
| ROBO1-UR | 5'-ccAaAcccttcctccAAaAc-3' | 20 bp |
| ROBO3-MF | 5'-gcgggaTtTtTagTcggTTT-3' | 20 bp |
| ROBO3-MR | 5'-gAcctctccgcaAActAAcg-3' | 20 bp |
| ROBO3-UF | 5'-TggTgggaTtTtTagTTggTTT-3' | 22 bp |
| ROBO3-UR | 5'-ccAcaActtccccAcAAcAc-3' | 20 bp |
| RT-PCR primers: | ||
| SLIT1-F | 5'-ctggaactcaatggcaacaa-3' | 20 bp |
| SLIT1-R | 5'-acaaagcctggttgttctgg-3' | 20 bp |
| SLIT2-F | 5'-tcagctgtttcctgagttgc-3' | 20 bp |
| SLIT2-R | 5'-tggttgaaacttgccacaga-3' | 20 bp |
| SLIT3-F | 5'-gcgcctgaacaagaataagc-3' | 20 bp |
| SLIT3-R | 5'-ggatgcgactgatgttgttg-3' | 20 bp |
| ROBO1-F1 | 5'-tgtttctggcccagcttatt-3' | 20 bp |
| ROBO1-R1 | 5'-gtgttcaacaatgcgaggtg-3' | 20 bp |
| ROBO1-F2 | 5'-aaatatggtgggcaaagctg-3' | 20 bp |
| ROBO1-R2 | 5'-ctggatgactgtggtggttg-3' | 20 bp |
| ROBO3-F | 5'-gcagtcctccgtgatgattt-3' | 20 bp |
| ROBO3-R | 5'-ttggaggctacgcacacata-3' | 20 bp |
| Cloning Primers: | ||
| SLIT2-cl-F | 5'-gaattTaaagTTtgggaaaagttg-3' | 24 bp |
| SLIT2-cl-R | 5'-cttccaacaactactaaaatacaaaaa-3' | 27 bp |
| SLIT2-cl-F3 | 5'-agtgTtgaTtagtggatatttTtgTT-3' | 26 bp |
| SLIT2-cl-R3 | 5'-tcttctAtctcccaaAAatAaactt-3' | 25 bp |
Nucleotides shown in upper case represent converted bases; MF and MR, methylation-specific forward and reverse primers; UF and UR, unmethylated forward and reverse primers; F and R, forward and reverse primers; cl-F and cl-R, cloning forward and reverse primers