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. Author manuscript; available in PMC: 2006 Jun 23.
Published in final edited form as: J Periodontol. 2005 Jul;76(7):1189–1201. doi: 10.1902/jop.2005.76.7.1189

Figure 1.

Figure 1

Characterization of cdt gene products produced by E. coli BL-21(DE3) (pET15bcdt). A) No bacterial extract or total soluble protein extract (10 ng of protein/μl of medium) from A. actinomycetemcomitans Y4, E. coli DH5α (pBluescript II SK+), E. coli DH5α (pCDT1), or E. coli BL-21(DE3) (pET15bcdt) was added to cultures of HeLa cells. *Statistically significant differences, P<0.05. B) SDS-PAGE of total soluble protein from recombinant strains of E. coli containing combinations or individual cdt genes cloned in pET15b. A, B, C: native polypeptides CdtB and CdtC; His: His-tagged polypeptides determined by Western blotting; *truncated CdtA.