Fig. 1.

Apo2L/TRAIL induces selective cleavage of the clathrin-dependent endocytosis machinery. (a) Cells were treated at 37°C with either trimeric Apo2L/TRAIL (Colo205 and HCT8) or antibody-crosslinked, tagged Apo2L/TRAIL (BJAB and HeLa-M), and cell lysates were analyzed by immunoblot for cleavage of caspase-8 (C8), caspase-3 (C3), adaptin (AP) 2α, AP1/2β (antibody does not distinguish the AP1 and 2 isoforms), CHC, or Tf receptor (TfR). (b) Colo205 cells were treated as in a and analyzed by immunoblot for processing of specific components of various types of clathrin-associated endocytic trafficking.