Fig. 2.

Involvement of different caspases in cleavage of AP2α and CHC. (a) BJAB cells were treated with the pan-caspase inhibitor zVAD-fmk (20 μM, 30 min) followed by treatment with crosslinked Apo2L/TRAIL (1 μg/ml) and analyzed by immunoblot for processing of caspase-8, caspase-3, AP2α, and CHC. Arrows with open heads indicate cleavage products, and arrows with filled heads indicate full-length proteins. (b–d) Bax^−/− or Bax^+/− HCT116 cells or caspase-3-defecient MCF-7 cells were treated with Apo2L/TRAIL and analyzed as in a.