Figure 1. Cholesterol efflux from human fibroblasts induced by Aramchol, apoA-I and BSA.

(A) Cultured human fibroblasts were loaded with [³H]cholesterol, and subsequent efflux of cholesterol in response to 2 μg/ml Aramchol, 2 mg/ml BSA or 5 μg/ml apoA-I, was determined, as described in the Materials and methods section. At the time points indicated, incubations were terminated. (B) Cholesterol efflux from human fibroblasts was performed using increasing concentrations of Aramchol in the presence of 2 mg/ml BSA. Efflux activity by the cells is given as percentage of the total radioactivity present in the cells at the onset of the experiment. The different incubations were carried out in triplicate and data are presented as means±S.D.