Table 1. Aramchol distribution in cells and medium after efflux.

Cultured human fibroblasts were loaded with cholesterol and subsequently efflux of cholesterol in response to 2 mg/ml BSA, 5 μg/ml apoA-I or 50 μg/ml HDL was determined in the presence of 2 μg/ml aramchol. Subsequently, aramchol was measured in cells and supernatant by LC–tandem MS as described in the Materials and methods section. Results are means±S.D. (n=6).

Treatment	Percentage in medium (mean±S.D.)	Recovery (%)
BSA	91±0.7	95
BSA, apoA-I	94±0.3	93
BSA, HDL	97±2.1	99.6