Figure 3. Reduction of E2F/DP activity causes decreased ASK-1 expression.

HeLa (0) and MCF10A cells were infected with retrovirus encoding DP1-shRNA or control shRNA. The amounts of endogenous DP1, DP2, ASK-1 and CCNA2 transcripts and ARPP-P0 transcripts as a control were determined by semi-quantitative RT–PCR analysis using RNA prepared 4 days after infection. The results shown are representative of three independent experiments.