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. 2006 May 29;396(Pt 3):547–556. doi: 10.1042/BJ20051981

Figure 8. Mutation of the ASK-1 promoter at positions −11 and −12 abolishes the E2F transactivation.

Figure 8

U2OS cells were transfected with 0.15 μg of expression vector (pcDNA3-HA control or the pcDNA3-HA-E2F and pcDNA3-HA-DP1 vectors) and 0.3 μg of the ASK-1 reporter construct H(−95/+11) wild-type or mutated in the putative E2F-binding site BS1 (M1) or BS2 (M2). The responsiveness of the various forms of ASK-1 promoter to E2F1 induction is presented relative to that of the empty pGL3-basic, arbitrarily set to 1.