Table 2. Oligonucleotides used in binding assays.
Name | Sequence |
---|---|
E2F-WT (wild-type) | 5′-ATTTAAGTTTCGCGCCCTTTCTCAA-3′ |
E2F (mutated) | 5′-ATTTAAGTTTCGATCCCTTTCTCAA-3′ |
A(−95/−72) | 5′-CGCCCTTCCTGGCCCCTCCCAGAG-3′ |
B(−74/−54) | 5′-GAGCGGCCGGGACGGAAGGAG-3′ |
C(−56/−20) | 5′-GAGCTGCGCGGCGCGGCGCGGCGCGGCGCGGCGGGTG-3′ |
D(−22/+11) | 5′-GTGGCGAGGGCGGGCTGCACCCCGAGCGCGGCG-3′ |
D-M1 | 5′-GTGGCGAGGGTAGGCTGCACCCCGAGCGCGGCG-3′ |
D-M2 | 5′-GTGGCGAGGGCGGGCTGCACCCCGAGCAAGGCG-3′ |
D-M1+2 | 5′-GTGGCGAGGGTAGGCTGCACCCCGAGCAAGGCG-3′ |