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Kd and Bmax values were derived from saturation binding experiments as described in the Experimental section. Values are the means±S.D. for three independent experiments.
| Protein | Kd (μM) | Bmax (μmol·g−1) |
|---|---|---|
| Wild-type | 1.4±0.1 | 41.1±1.1 |
| Y32A | 0.8±0.1 | 23.4±0.7 |
| W47A | 4.4±0.6 | 23.0±1.3 |
| Y32A/W47A | 25.0±2.9 | 5.1±0.3 |
