Table 2. Affinity of RoGACBM21 derivatives for soluble oligosaccharides determined by fluorescence titration spectra.

Values are the means±S.D. for three independent titrations. No binding was detected in the case of protein W47A.

		Kd (μM)
Ligands	Protein …	Wild-type	Y32A
γCD		8.3±1.5	6.6±0.8
βCD		5.1±0.7	6.0±0.7
αCD		333.0±13.1	286.3±30.5
Maltoheptaose		5.5±1.0	44.7±5.3
Maltohexaose		24.4±1.7	46.4±5.8
Maltopentaose		27.5±1.4	50.2±6.2
Maltotetraose		54.4±4.8	53.3±5.5
Maltotriose		61.9±6.0	58.2±8.1
Isomaltotrisoe		3.8±0.7	3.9±0.8

NB, no binding detected.