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. 2006 May 29;396(Pt 3):565–571. doi: 10.1042/BJ20060038

Figure 2. Formation of a complex between Ph-P0 and Ph-L12.

Figure 2

(A) Isolated Ph-L12 (600 pmol, lane 1), Ph-P0 (100 pmol, lane 2) and the complex (lane 3) formed with both proteins was subjected to non-denaturing gel electrophoresis. The gel was stained with Coomassie Brilliant Blue R-250. The band that appeared as a result of mixing the two proteins is indicated with an arrow. (B) Major bands that appeared in (A), lanes 1, 2 and 3 (arrow), were cut out of the gel, incubated in SDS sample solution and then used as samples for SDS/gel electrophoresis. The gel was stained with the fluorescent reagent SYPRO Orange (Molecular Probes, Invitrogen) and the pattern was visualized with a STORM 860 PhosphorImager (Amersham Biosciences).