Figure 4. Binding ability of the P. horikoshii ribosomal proteins to the 1070 rRNA fragment.

An aliquot (20 pmol) of each of isolated Ph-P0 (lane 2), Ph-L12 (lane 3), Ph-L11 (lane 4), Ph-P0+Ph-L11 (lane 5), Ph-P0·Ph-L12 complex (lane 6), and Ph-P0·Ph-L12+Ph-L11 (lane 7) was mixed with 5 pmol of the [³²P]RNA fragment covering the 1070 domain of P. horikoshii 23S rRNA (lane 1, RNA alone), separated by native gel electrophoresis and then subjected to autoradiography.