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An aliquot (10 pmol) of the 50S core particles deficient in L10·L7/L12 and L11 (lane 1) was incubated with 20 pmol of Ph-P0 (lane 2), Ph-L12 (lane 3), Ph-L11 (lane 4), Ph-P0+Ph-L11 (lane 5), the Ph-P0·Ph-L12 complex (lane 6), and Ph-P0·Ph-L12+Ph-L11 (lane 7), and then analysed by acrylamide/agarose composite gel electrophoresis, as described in the Experimental section.
