Figure 7. The GTPase activity of the hybrid ribosomes dependent on P. horikoshii EF-2 (A), pig eEF-2 (B) and E. coli EF-G (C).

E. coli 50S core particles (2.5 pmol) deficient in L10, L7/L12 and L11 were pre-incubated with 10 pmol of each of L10·L7/L12 and L11 (Eco). The 50S cores were also incubated with 10 pmol of each of Ph-P0·Ph-L12, and Ph-L11 (Ph) or silkworm P0·P1-P2 and eL12 (Bm). The resulting 50S particle samples were assayed for GTPase activity in the presence of E. coli 30S subunits (7.5 pmol).