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IEF (pH 3.5–10) followed by in-gel activity detected by the formation of a whitish precipitate resulting from the reaction of calcium, with Pi released from ATP (lanes 1 and 2) and ADP (lanes 3 and 4), but not AMP (lanes 5 and 6). These samples were applied 1.5 cm from the activity area on the basic (lanes 1, 3 and 5) or acidic (lanes 2, 4, and 6) sides of the gel. Arrows indicate positions of focused pI standards.
