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Purified apyrases were submitted to non-reducing SDS/6% PAGE, then the gel lane was cut out, and disulphide bonds were reduced by incubation in reducing sample buffer. The lane was then applied horizontally on top of a 6% gel and run into a second dimension. Upper and left-hand lanes represent controls run only in the first non-reducing dimension (1st dim.) and the second reducing dimension (2d dim.) respectively.
