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Purified apyrases were incubated with increasing concentrations of DTSSP (0, 1, 2.5, 5, 10 and 25 μM) and submitted to non-reducing SDS/6% PAGE. The position of molecular-mass standards is indicated by arrows: myosin, 212 kDa; α-macroglobulin, 170 kDa; β-galactosidase, 116 kDa and transferrin, 76 kDa.
