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. 2006 May 29;396(Pt 3):479–485. doi: 10.1042/BJ20051711

Figure 5. HICDH-coupled assay of the conversion of (R)-homocitrate into (2R,3S)-homoisocitrate into the presence of 2.5 μg/ml HACN (■), 70 μg/ml ACN (○), and ACN+HACN (●).

Figure 5

Conditions: 50 mM Hepes/NaOH (pH 8.0), 200 mM KCl, 5 mM MgCl2, 1 mM NAD+ and 29 μg/ml HICDH, at 40 °C. AU, arbitrary units.