Table 2. Interaction of Oregon-Green-labelled gelsolin and gelsolin domains with calponin h1 was monitored by Oregon Green fluorescence.
Changes in the intensity of the fluorescence emission spectra were recorded at various calponin concentrations in 0.1 M Tris/HCl buffer, pH 7.5, supplemented with 1 mM EGTA. Percentage fluorescence decreases were extrapolated to infinite calponin concentration.
| Kd (nM) | Fluorescence decrease (%) | Stoichiometry | |
|---|---|---|---|
| Gelsolin | 16±4 | 45±5 | 1 |
| G1–G3 | 20±7 | 44±5 | 1 |
| G4–G6 | 25±8 | 33±5 | 1 |