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. 2006 May 29;396(Pt 3):411–417. doi: 10.1042/BJ20051814

Figure 6. MEX binds with UbcH5a as an E2.

Figure 6

(A) Wild-type (WT) and deletion mutants of MEX proteins. (B) HEK-293T cells were co-transfected with the indicated FLAG-tagged MEX and the Myc-tagged UbcH5a constructs. Cells were treated as described in Figure 3(A) using an anti-Myc Ab (top panel) for immunoblotting (IB). Total lysates were blotted with an anti-Myc (middle panel) or an anti-FLAG Ab (bottom panel). (C) HEK-293T cells were co-transfected with the indicated constructs and protein interactions were analysed as described in (B). IP, immunoprecipitate.