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. 2006 May 29;396(Pt 3):411–417. doi: 10.1042/BJ20051814

Figure 7. MEX self-association is independent of the SWIM domain.

Figure 7

(A) HEK-293T cells were co-transfected with FLAG-tagged MEX and the indicated Myc-tagged MEX constructs. Cells were treated as described in Figure 3(A) using an anti-Myc Ab (top panel) for immunoblotting (IB). Total lysates were blotted with an anti-Myc (middle panel) or an anti-FLAG Ab (bottom panel). (B) HEK-293T cells were co-transfected with the Myc-tagged MEX mutant (amino acids 139–398) construct and the indicated FLAG-tagged MEX constructs. Protein interactions were analysed as described in (A). IP, immunoprecipitate.