Table 2.
Key refinement statistics for three Complex II structures used in this work. Additional statistics from the data processing and refinement work are available in supplemental materials.
| PDB ID code | 1YQ3 | 1YQ4 | 9ZZZ |
|---|---|---|---|
| Added Ligand | OAA | 3-NP | Carboxin |
| Space Group | P212121 | P212121 | P21 |
| Cell parameters | 70.0 × 84.4 × 289.5 | 69.6 × 83.5 × 288.6 | 122.0 × 203.4 × 69.0, 90.0 90.0 90.0 |
| Resolution range (last shell) | 8.66−2.20 2.24−2.19) | 56.38 − 2.33 (2.33 – 2.38) | 34.87 − 2.10 (2.1–2.15) |
| Completeness | 89.2% (47.1%) | 93.9% (80.2%) | 86.0 (42.8) |
| # Reflections | 78719 (2623) | 68501 (3672) | 166872 (5572) |
| Cryst. R Value | 0.176 (0.267) | 0.205 (0.32) | 0.195 (0.30) |
| Free R Value | 0.225 (0.313) | 0.259 (0.38) | 0.235 (0.36) |
| B Values | |||
| From Wilson Plot | 34.4 Å2 | 30.9 Å2 | 11.9 Å2 |
| Mean atomic B Val | 47.8 Å2 | 47.1 Å2 | 31.3 Å2 |
| RMS Deviations from Ideality: | |||
| Bond Lengths | 0.022 Å | 0.019 Å | 0.032 |
| Bond Angles | 1.8° | 1.9° | 2.0° |
| Dihedral Angles | 22.4° | 22.3° | 22.8 |
| Improper Angles | 1.02° | 1.08° | 1.15 |