TABLE 2.
Effect of the SBD1 allele on expression of a yitJ-lacZ transcriptional fusion and growth
| Strain | metB | Methionine addition | yitJ-lacZ expressiona | Doubling time (min) |
|---|---|---|---|---|
| BR151 | − | − | 120 | NDb |
| − | + | 0.38 | 58 | |
| BR151-SBD1 | − | − | 180 | ND |
| − | + | 50 | 60 | |
| BR151MA | + | − | 64 | 84 |
| + | + | 0.14 | 76 | |
| BR151MA-SBD1 | + | − | 44 | 130 |
| + | + | 40 | 134 |
a
Cells of strains BR151 and BR151-SBD1 (metB10) or BR151MA and BR151MA-SBD1 (Met+) were grown in minimal medium containing methionine, harvested by centrifugation, and resuspended in minimal medium in the presence (+) or absence (−) of methionine. yitJ-lacZ fusion activity is in Miller units (22) and is reported for the time points at which maximal expression was observed (4 h after the cultures were split for BR151 and BR151-SBD1 and 1 h after the cultures were split for BR151MA and BR151MA-SBD1).
b
ND, not determined.