TABLE 2.
DNA primers used in this study
| Primer | 5′-3′ sequence (restriction endonuclease)a |
|---|---|
| CS_for | GGCTCATGAGCCTCAGATATACCCGTAATGC (BspHI) |
| CS_rev | GCTCTAGAAGAGCAGCCGCTAAGTAAAACCAG (XbaI) |
| CD_rev | GCTCTAGAATCGCAGCCGCTAAGTAAAAC (XbaI) |
| RFP_for | GCTCTAGAGCCTCCTCCGAGGACGTCATC (XbaI) |
| RFP_rev | CCCAAGCTTAGGCGCCGGTGGAGTGG (HindIII) |
| F_mut_for | GGAAAAGCAGCCGCTAAGTAAAACCAG |
| Mut_rev | AGAGCCTCCTCCGAGGACGTC |
| FN_mut_for | GATCGCTGGTTTTACTTAGCGGCTGCTTTAACTCC |
| Xba-RFP-5 | CTAGTCTAGAATGGCCTCCTCC (XbaI) |
| HindIIIRFP-3 | CCTGAAGCTTTTAGGCGCCGGTGGAGTGGCGG (HindIII) |
a
Restriction endonuclease cleavage sites are underlined.