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Epistatic effects of mutant Aer proteins on Tar and Tsr function. Plasmids (pNP1 [Aer vector control], pKG106 [wild-type Aer], pKG106-F363W, and pKG106-N366W) were introduced into strains expressing only Tsr (UU1615) or Tar (UU1624) from the chromosome. Transformant cells were tested for serine chemotaxis (32°C) or aspartate chemotaxis (30°C) on tryptone soft agar after incubation for the following times: (A) 7 h, (B) 12 h, (C) 16 h, and (D) 16 h. WT, wild type.
