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Phenotypic suppression of CW-biased signaling defects in the Aear-269 chimeric transducer. Strains containing plasmids with CW-biased mutations in Aer (pKG106 derivatives [A and C]) or Aear-269 (pKG122 derivatives [B and D]) were tested for aerotactic ability on tryptone soft agar (50 μM IPTG) at 30°C. Incubation times were 17 h (A, C, and D) and 12 h (B). WT, wild type; R+ B+, strain with CheR/CheB functions; R− B−, strain without CheR/CheB functions.
