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Localization of TcdC and ATPase in membrane fractions collected after sucrose density gradient centrifugation. Lanes are marked with the collected-fraction number. (A) Proteins were stained with Coomassie brilliant blue. (B) TcdC was revealed by anti-TcdC antibody. (C) Immunoblot using anti-ATP synthetase β-subunit antibody (dilution, 1:50,000).
