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Genetic requirements for growth of B. subtilis 168 in FS-MM with or without citrate. (A) In FS-MM lacking citrate, good growth was observed for CU1065 (▪) and the isogenic yfmC mutant (•). The dhbA mutant (▴) grows somewhat more slowly, and the ywbL mutant (▵) fails to grow. (B) In the presence of citrate, all strains grow well with the exception of the yfmC mutant. Growth was assessed by using 96-well microtiter plates as described in Materials and Methods.
