TABLE 1.
Strains used in this study
| Strain | Genotype (siderophore)a | Source or referenceb |
|---|---|---|
| CU1065 | trpC2 attSPβ sfp0 [DHB(G)+] | Lab stock |
| NCIB3610 | sfp+ (bacillibactin+) | 10 |
| HB2501 | CU1065 fur::kan [DHB(G)+] | 19 |
| HB5600 | CU1065 dhbA::spc | LFH-PCR → CU1065 |
| HB5601 | CU1065 dhbA::mls | LFH-PCR → CU1065 |
| HB5602 | CU1065 fhuD::spc [DHB(G)+] | LFH-PCR → CU1065 |
| HB5604 | CU1065 yclN::mls [DHB(G)+] | LFH-PCR → CU1065 |
| HB5605 | CU1065 yclN::mls dhbA::spc | HB5600 chr DNA → HB5604 |
| HB5606 | CU1065 yhfQ::cat [DHB(G)+] | LFH-PCR → CU1065 |
| HB5607 | CU1065 yhfQ::cat dhbA::spc | HB5600 chr DNA → HB5606 |
| HB5608 | CU1065 fhuB::mls [DHB(G)+] | LFH-PCR → CU1065 |
| HB5609 | CU1065 fhuB::mls dhbA::spc | HB5600 chr DNA → HB5608 |
| HB5610 | CU1065 yusV::mls [DHB(G)+] | LFH-PCR → CU1065 |
| HB5611 | CU1065 yusV::mls dhbA::spc | HB5600 chr DNA → HB5610 |
| HB5612 | CU1065 yfmC::mls [DHB(G)+] | LFH-PCR → CU1065 |
| HB5613 | CU1065 yfmC::mls dhbA::spc | HB5600 chr DNA → HB5612 |
| HB5614 | CU1065 yuiI::spc [DHB(G)+] | LFH-PCR → CU1065 |
| HB5615 | CU1065 yuiI::spc dhbA::mls | HB5601 chr DNA → HB5614 |
| HB5616 | CU1065 yfiY::spc [DHB(G)+] | LFH-PCR → CU1065 |
| HB5617 | CU1065 yfiY::spc dhbA::mls | HB5601 chr DNA → HB5616 |
| HB5618 | CU1065 yfiZ::cat [DHB(G)+] | LFH-PCR → CU1065 |
| HB5619 | CU1065 yfiZ::cat dhbA::mls | HB5601 chr DNA → HB5618 |
| HB5620 | CU1065 ywjA::spc [DHB(G)+] | LFH-PCR → CU1065 |
| HB5621 | CU1065 ywjA::spc dhbA::mls | HB5601 chr DNA → HB5620 |
| HB5622 | CU1065 ywbL::cat [DHB(G)+] | LFH-PCR → CU1065 |
| HB5623 | CU1065 ywbL::cat dhbA::spc | HB5600 chr DNA → HB5622 |
| HB5624 | CU1065 feuA::spc [DHB(G)+] | LFH-PCR → CU1065 |
| HB5625 | CU1065 feuA::spc dhbA::mls | HB5601 chr DNA → HB5624 |
| HB5626 | CU1065 yxeB::spc [DHB(G)+] | LFH-PCR → CU1065 |
| HB5628 | CU1065 yfhA::spc [DHB(G)+] | LFH-PCR → CU1065 |
| HB5630 | CU1065 yusV::mls feuA::spc [DHB(G)+] | HB5610 chr DNA → HB5624 |
| HB5800 | sfp+ (prototroph) (bacillibactin+) | OKB105 chr DNA → CU1065 |
| HB5700 | HB5800 dhbA::spc | HB5600 chr DNA → HB5800 |
| HB5701 | HB5800 dhbA::mls | HB5601 chr DNA → HB5800 |
| HB5712 | HB5800 yfmC::mls (bacillibactin+) | HB5612 chr DNA → HB5800 |
| HB5722 | HB5800 ywbL::cm (bacillibactin+) | HB5622 chr DNA → HB5800 |
| HB5710 | HB5800 yusV::mls | HB5610 chr DNA → HB5800 |
| HB5714 | HB5800 yuiI::spc | HB5614 chr DNA → HB5800 |
| HB5724 | HB5800 feuA::spc | HB5624 chr DNA → HB5800 |
| HB5730 | CU1065 amyE::PycgT-lacZ(cat) | See Materials and Methods |
| HB5731 | CU1065 fur::kan amyE::PycgT-lacZ(cat) | See Materials and Methods |
The siderophores produced by each strain are indicated. Only sfp+ strains produce bacillibactin. DHB(G) refers to the mixture of 2,3-dihydroxybenzoic acid and its glycine conjugate (Itoic acid) produced by 168 (Sfp0) strains that are not mutated at the dhb locus. Except where indicated, all gene disruptions were double-crossover, allelic replacements using the indicated antibiotic cassettes: kan, kanamycin resistance; spc, spectinomycin resistance; mls, macrolide-lincomycin streptogramin B resistance; cat, chloramphenicol resistance.
Some strains were constructed by long-flanking homology PCR (LFH-PCR) or by transformation of chromosomal DNA (chr DNA) from one donor strain into the indicated recipient with selection for the antibiotic marker associated with the donor DNA.