FIG. 2.

PAGE analyses of rhamnan-containing LPSs present in the aqueous or phenol phase of hot phenol-water extracts of Rhizobium sp. strain NGR234 (and various mutants thereof). The gels were silver stained for LPS. Lanes contain extracts from the following sources: 1, noninduced wild-type NGR234 (grown in the absence of apigenin); 2, apigenin-induced (IND) NGR234; 3, IND NGRΔrmlB-wbgA; 4, IND NGRΩfixF; 5, IND NGRΩrmlC; 6, IND NGRΩgM; 7, IND NGRΩhA; 8, IND NGRΩnoeE. The position of the rhamnan O antigen produced by induced NGR234 and certain derivatives is indicated. The position of the R-LPS produced by all the strains and present in both phases is also marked. Finally the vhm-R-LPS produced by certain mutants is indicated.