TABLE 3.
Effects of disruption of open reading frames between NB5 and NB7 on nodulation capacity of Rhizobium sp. strain NGR234
| Plantd | Phenotype after inoculation witha:
|
||||||
|---|---|---|---|---|---|---|---|
| NGR234 | NGRΔ rmlB-wbgAb | NGRΩfixF | NGRΩrmlC | NGRΩgM | NGRΩhA | NGRΔnoeE | |
| L. leucocephala (I) | Fix+ | Fix+ | Fix+ | Fix+ | Fix+ | Fix+ | Fix+ |
| M. atropurpureum (D) | Fix+ | Fix+/− | Fix− | Fix+ | Fix− | Fix+ | Fix+ |
| P. tuberosusc (D) | Nod− | Nod− | Nod− | Nod− | Nod− | Nod− | Nod− |
| T. vogelii (I) | Fix+ | Fix− | Fix− | Fix+ | Fix− | Fix+ | Fix+ |
| V. unguiculatan (D) | Fix+ | Fix+/− | Fix− | Fix+ | Fix− | Fix+ | Fix+ |
Results are the means of at least three independent experiments (four Magenta jars per treatment, four plants per jar). Plants were harvested 6 to 8 weeks after inoculation and assessed qualitatively. Leaf color, shoot dry weight, and the presence (or absence) of fixing (pink) nodules per plant were determined at harvest. Inoculated plants with yellow leaves and dry weights similar to those of uninoculated plants were classified as Fix−. Inoculated plants with greenish-yellow leaves and intermediate dry weights were scored as Fix+/−. Fix+ signifies dark green leaves and dry weights equivalent to those of plants inoculated with wild-type NGR234. Nod− means that nodules were not formed.
See text for further description of this inoculant.
P. tuberosus occasionally produced a nodule after inoculation, regardless of the strain, but generally roots appeared uninfected (see text).
dI or D, indeterminate or determinate nodules.